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The discrepancy between what was expected and what has been observed has grown over the years, and we're straining harder and harder to fill the gap.
Biology has tended to be an observational science and deriving things from first principles has not been possible in the past but I hate to predict the future on that.
There are reasons, increasing in number and quality, to believe that the masses of ordinary galaxies may have been underestimated by a factor of 10 or more.